Shaw, M;
Elmi, M;
Pawar, V;
Srinivasan, MA;
(2016)
Investigation of mechanosensation in C. elegans using light field calcium imaging.
Biomedical Optics Express
, 7
(7)
pp. 2877-2887.
10.1364/BOE.7.002877.
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Abstract
We describe a new experimental approach to investigate touch sensation in the model organism C. elegans using light field deconvolution microscopy. By combining fast volumetric image acquisition with controlled indentation of the organism using a high sensitivity force transducer, we are able to simultaneously measure activity in multiple touch receptor neurons expressing the calcium ion indicator GCaMP6s. By varying the applied mechanical stimulus we show how this method can be used to quantify touch sensitivity in C. elegans. We describe some of the challenges of performing light field calcium imaging in moving samples and demonstrate that they can be overcome by simple data processing.
| Type: | Article |
|---|---|
| Title: | Investigation of mechanosensation in C. elegans using light field calcium imaging |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1364/BOE.7.002877 |
| Publisher version: | http://doi.org/10.1364/BOE.7.002877 |
| Language: | English |
| Additional information: | Copyright © 2017 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Optics, Radiology, Nuclear Medicine & Medical Imaging, Biochemistry & Molecular Biology, Caenorhabditis-elegans, Pacinian Corpuscle, Neuronal-activity, Touch Sensation, Channel, Microscope, Mechanotransduction, Subunit, Protein, Model |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1506360 |
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