Stricker, SH;
Koferle, A;
Beck, S;
(2017)
From profiles to function in epigenomics.
[Review].
Nature Reviews Genetics
, 18
(1)
pp. 51-66.
10.1038/nrg.2016.138.
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Abstract
Myriads of epigenomic features have been comprehensively profiled in health and disease across cell types, tissues and individuals. Although current epigenomic approaches can infer function for chromatin marks through correlation, it remains challenging to establish which marks actually have causative roles in gene regulation and other processes. After revisiting how classical approaches have addressed this question in the past, we discuss the current state of epigenomic profiling and how functional information can be indirectly inferred. We also present new approaches that promise definitive functional answers, which are collectively referred to as 'epigenome editing'. In particular, we explore CRISPR-based technologies for single-locus and multi-locus manipulation. Finally, we discuss which level of function can be achieved with each approach and introduce emerging strategies for high-throughput progression from profiles to function.
| Type: | Article |
|---|---|
| Title: | From profiles to function in epigenomics |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/nrg.2016.138 |
| Publisher version: | http://dx.doi.org/10.1038/nrg.2016.138 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Genetics & Heredity, Targeted Dna Methylation, De-Novo Methylation, Epigenetic Regulation, Gene-Expression, Transcriptional Regulation, Histone Deacetylase-1, Mammalian Development, Cell Differentiation, Early Embryogenesis, Lysine Methylation |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1530706 |
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