Malecki, M;
Bähler, J;
(2016)
Identifying genes required for respiratory growth of fission yeast.
Wellcome Open Research
, 1
, Article 12. 10.12688/wellcomeopenres.9992.1.
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Abstract
We have used both auxotroph and prototroph versions of the latest deletion-mutant library to identify genes required for respiratory growth on solid glycerol medium in fission yeast. This data set complements and enhances our recent study on functional and regulatory aspects of energy metabolism by providing additional proteins that are involved in respiration. Most proteins identified in this mutant screen have not been implicated in respiration in budding yeast. We also provide a protocol to generate a prototrophic mutant library, and data on technical and biological reproducibility of colony-based high-throughput screens.
| Type: | Article |
|---|---|
| Title: | Identifying genes required for respiratory growth of fission yeast |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.12688/wellcomeopenres.9992.1 |
| Publisher version: | http://dx.doi.org/10.12688/wellcomeopenres.9992.1 |
| Language: | English |
| Additional information: | Copyright © 2016 Malecki M and Bähler J. This is an open access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Respiration, energy metabolism, fermentation, fission yeast, functional profiling, glycerol, mitochondria, mutant screen |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1533217 |
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