Miller, A-M;
Rutkowska, A;
Bahl, JM;
Herukka, S-K;
Koel-Simmelink, MJA;
Kruse, N;
Mollenhauer, B;
... Lawlor, BA; + view all
(2016)
Multicenter immunoassay validation of cerebrospinal fluid neurofilament light: a biomarker for neurodegeneration.
Bioanalysis
, 8
(21)
pp. 2243-2254.
10.4155/bio-2016-0114.
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Abstract
AIM: Neurofilament light (NfL) chain, a putative cerebrospinal fluid biomarker, can support neurodegenerative disease diagnosis and indicate disease severity and prognosis. Universal validation protocols when used to measure biomarkers can reduce pre and analytical laboratory variation, thus increasing end-user confidence in the consistency of validation data across sites. METHODOLOGY: Here, a commercially available NfL ELISA (UmanDiagnostics, Umeå, Sweden) was validated in a multicentered setting using comprehensive newly developed standard operating procedures. RESULTS: The data showed good assay sensitivity and intra and interassay precision. Interlaboratory precision was, however, suboptimal. CONCLUSION: The UmanDiagnostics assay is suitable for the quantification of NfL in human cerebrospinal fluid. However, sources of interlaboratory variation in the data require further investigation.
| Type: | Article |
|---|---|
| Title: | Multicenter immunoassay validation of cerebrospinal fluid neurofilament light: a biomarker for neurodegeneration |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.4155/bio-2016-0114 |
| Publisher version: | http://dx.doi.org/10.4155/bio-2016-0114 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Physical biomarker, validation, neurofilament light (NfL), cerebrospinal fluid (CSF), multi-centre |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neurodegenerative Diseases |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1537957 |
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