Lujan, BJ;
Roorda, A;
Croskrey, JA;
Dubis, AM;
Cooper, RF;
Bayabo, J-K;
Duncan, JL;
... Carroll, J; + view all
(2015)
Directional optical coherence tomography provides accurate outer nuclear layer and Henle fiber layer measurements.
Retina
, 35
(8)
pp. 1511-1520.
10.1097/IAE.0000000000000527.
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Abstract
Purpose: The outer nuclear layer (ONL) contains photoreceptor nuclei, and its thickness is an important biomarker for retinal degenerations. Accurate ONL thickness measurements are obscured in standard optical coherence tomography (OCT) images because of Henle fiber layer (HFL). Improved differentiation of the ONL and HFL boundary is made possible by using directional OCT, a method that purposefully varies the pupil entrance position of the OCT beam. Methods: Fifty-seven normal eyes were imaged using multiple pupil entry positions with a commercial spectral domain OCT system. Cross-sectional image sets were registered to each other and segmented at the top of HFL, the border of HFL and the ONL and at the external limiting membrane. Thicknesses of the ONL and HFL were measured and analyzed. Results: The true ONL and HFL thicknesses varied substantially by eccentricity and between individuals. The true macular ONL thickness comprised an average of 54.6% of measurements that also included HFL. The ONL and HFL thicknesses at specific retinal eccentricities were poorly correlated. Conclusion: Accurate ONL and HFL thickness measurements are made possible by the optical contrast of directional OCT. Distinguishing these individual layers can improve clinical trial endpoints and assessment of disease progression.
| Type: | Article |
|---|---|
| Title: | Directional optical coherence tomography provides accurate outer nuclear layer and Henle fiber layer measurements |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1097/IAE.0000000000000527 |
| Publisher version: | http://dx.doi.org/10.1097/IAE.0000000000000527 |
| Language: | English |
| Additional information: | Copyright © 2015. This manuscript version is published under a Creative Commons Attribution Non-commercial 4.0 International licence (CC BY-NC 4.0). This licence allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licences are available at http://creativecommons.org/licenses/by/4.0. Access may be initially restricted by the publisher. |
| Keywords: | spectral domain; Fourier domain; directional reflectivity; outer plexiform layer; Henle's fiber layer; photoreceptors; cones; rods; biomarker |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1543199 |
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