da Costa, NM;
de Siqueira, AS;
Ribeiro, AL;
da Silva Kataoka, MS;
Jaeger, RG;
de Alves-Júnior, SM;
Smith, AM;
(2017)
Role of HIF-1α and CASPASE-3 in cystogenesis of odontogenic cysts and tumors.
Clinical Oral Investigations
, 22
(1)
pp. 141-149.
10.1007/s00784-017-2090-6.
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Abstract
OBJECTIVES: Odontogenic cysts and tumors are the most relevant lesions that affect the gnathic bones. These lesions have in common the formation of cystic areas and this common feature may suggest involvement of similar mechanisms. The hypoxia inducible factor 1 alpha (HIF-1α), a responsive protein to hypoxia and caspase-3, an irreversible apoptosis marker, may contribute to cyst formation. Thus, this study aimed to investigate the immunoexpression of these proteins in odontogenic cysts and tumors. MATERIAL AND METHODS: Twenty cases of ameloblastoma, keratocystic odontogenic tumor (KOT) (n = 20), radicular cyst (RC) (n = 18), dentigerous cyst (DC) (n = 11), calcifying cystic odontogenic tumor (n = 8), and dental follicle (DF) (n = 10) were used to investigate HIF-1α and caspase-3 expression in sequential serial cuts by immunohistochemistry. RESULTS: HIF-1α was overexpressed in RC, DC, and ameloblastoma when compared with DF. The basal and sometimes the lower suprabasal layer showed no or very low expression in DC, KOT, and ameloblastoma, the last also showing strong expression in solid epithelial areas and initial cystic formation regions. Caspase-3 was found to be overexpressed in all lesions, with the highest expression in odontogenic cysts compared to tumors. HIF-1α and caspase-3 were localized in similar areas of the same lesions, especially in the epithelium surrounding cystic formations. CONCLUSIONS: This study showed distinct immunoexpression of HIF-1α and caspase-3 in odontogenic cyst and tumors, with higher expression observed in odontogenic cysts. CLINICAL RELEVANCE: These findings suggest a possible correlation between hypoxia, apoptosis, and cystogenesis, leading to understand the mechanisms responsible to cystic formation in odontogenic lesions.
| Type: | Article |
|---|---|
| Title: | Role of HIF-1α and CASPASE-3 in cystogenesis of odontogenic cysts and tumors |
| Location: | Germany |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s00784-017-2090-6 |
| Publisher version: | http://doi.org/10.1007/s00784-017-2090-6 |
| Language: | English |
| Additional information: | © Springer-Verlag Berlin Heidelberg 2017. The final publication is available at Springer via http://dx.doi.org/10.1007/s00784-017-2090-6 |
| Keywords: | Caspase-3, Cystogenesis, HIF-1α, Odontogenic cyst, Odontogenic tumors |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1544027 |
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