Martinez-Jimenez, CP;
Eling, N;
Chen, H-C;
Vallejos, CA;
Kolodziejczyk, AA;
Connor, F;
Stojic, L;
... Odom, DT; + view all
(2017)
Aging increases cell-to-cell transcriptional variability upon immune stimulation.
Science
, 355
(6332)
pp. 1433-1436.
10.1126/science.aah4115.
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Abstract
Aging is characterized by progressive loss of physiological and cellular functions, but the molecular basis of this decline remains unclear. We explored how aging affects transcriptional dynamics using single-cell RNA sequencing of unstimulated and stimulated naïve and effector memory CD4+ T cells from young and old mice from two divergent species. In young animals, immunological activation drives a conserved transcriptomic switch, resulting in tightly controlled gene expression characterized by a strong up-regulation of a core activation program, coupled with a decrease in cell-to-cell variability. Aging perturbed the activation of this core program and increased expression heterogeneity across populations of cells in both species. These discoveries suggest that increased cell-to-cell transcriptional variability will be a hallmark feature of aging across most, if not all, mammalian tissues.
| Type: | Article |
|---|---|
| Title: | Aging increases cell-to-cell transcriptional variability upon immune stimulation |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1126/science.aah4115 |
| Publisher version: | http://doi.org/10.1126/science.aah4115 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1555510 |
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