Clark, RE;
Dodi, IA;
Hill, SC;
Lill, JR;
Aubert, G;
Macintyre, AR;
Rojas, J;
... Madrigal, JA; + view all
(2001)
Direct evidence that leukemic cells present HLA-associated immunogenic peptides derived from the BCR-ABL b3a2 fusion protein.
Blood
, 98
(10)
pp. 2887-2893.
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Abstract
The BCR-ABL oncogene is central in the pathogenesis of chronic myeloid leukemia (CML). Here, tandem nanospray mass spectrometry was used to demonstrate cell surface HLA- associated expression of the BCR-ABL peptide KQSSKALQR on class I-negative CIVIL cells transfected with HLA-A*0301, and on primary CIVIL cells from HLA-A3-positive patients. These patients mounted a cytotoxic T-lymphocyte response to KQSSKALQR that also killed autologous CML cells, and tetramer staining demonstrated the presence of circulating KQSSKALQR-specific T cells. The findings are the first demonstration that CML cells express HLA-associated leukemia-specific immunogenic peptides and provide a sound basis for immunization studies against BCR- ABL. (C) 2001 by The American Society of Hematology
| Type: | Article |
|---|---|
| Title: | Direct evidence that leukemic cells present HLA-associated immunogenic peptides derived from the BCR-ABL b3a2 fusion protein |
| Open access status: | An open access version is available from UCL Discovery |
| Additional information: | Journal English Article AMER SOC HEMATOLOGY NOV 15 492PW WASHINGTON BLOOD 1900 M STREET. NW SUITE 200, WASHINGTON, DC 20036 USA |
| Keywords: | American, autologous, BCR-ABL, BCR-ABL ONCOGENE, Blood, cell, Cell Surface, CELL-SURFACE, CELLS, Chronic, CHRONIC MYELOGENOUS LEUKEMIA, CHRONIC MYELOID-LEUKEMIA, circulating, Class, CLASS-I MOLECULES, CML, cytotoxic T lymphocyte, CYTOTOXIC T-LYMPHOCYTES, Dendritic Cells, English, Epitopes, expression, FUSION, FUSION PROTEIN, Hematology, HLA, Immunization, Leukemia, LIFE, M, MASS, Mass Spectrometry, MASS-SPECTROMETRY, MYELOID-LEUKEMIA, NORMAL DONORS, ONCOGENE, PATHOGENESIS, Patient, patients, peptide, Peptides, PERIPHERAL-BLOOD, PHILADELPHIA-CHROMOSOME, PHYS, PROTEIN, response, societies, SOCIETY, sound, SPECTROMETRY, SPONTANEOUS REMISSION, Staining, SURFACE, T cell, T CELLS, T lymphocyte, T-CELL, T-CELLS, USA |
| UCL classification: | UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/6966 |
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