Heintze, J;
Costa, JR;
Weber, M;
Ketteler, R;
(2016)
Ribose 5-phosphate isomerase inhibits LC3 processing and basal autophagy.
Cellular Signalling
, 28
(9)
pp. 1380-1388.
10.1016/j.cellsig.2016.06.015.
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Abstract
Autophagy and cellular metabolism are tightly linked processes, but how individual metabolic enzymes regulate the process of autophagy is not well understood. This study implicates ribose-5-phosphate isomerase (RPIA), a key regulator of the pentose phosphate pathway, in the control of autophagy. We used a dual gene deletion strategy, combining shRNA-mediated knockdown studies with CRISPR/Cas9 genome editing. Knockdown of RPIA by shRNA or genomic deletion by CRISPR/Cas9 genome editing, results in an increase of ATG4B-mediated LC3 processing and in the appearance of LC3-positive autophagosomes in cells. Increased LC3 processing upon knockdown of RPIA can be reversed by treatment with the antioxidant N-acetyl cysteine. The results are consistent with a model in which RPIA suppresses autophagy and LC3 processing by modulation of redox signaling.
| Type: | Article |
|---|---|
| Title: | Ribose 5-phosphate isomerase inhibits LC3 processing and basal autophagy |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.cellsig.2016.06.015 |
| Publisher version: | http://dx.doi.org/10.1016/j.cellsig.2016.06.015 |
| Language: | English |
| Additional information: | © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Autophagy, CRISPR, Cas9, Pentose phosphate pathway, RPIA, shRNA |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1502406 |
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