Personne, Y;
Ozongwu, C;
Platt, G;
Basurto-Lozada, P;
Shamin, M;
Gant, VA;
Zumla, A;
(2016)
'Sample-in, answer-out'? Evaluation and comprehensive analysis of the Unyvero P50 pneumonia assay.
Diagnostic Microbiology and Infectious Disease
, 86
(1)
pp. 5-10.
10.1016/j.diagmicrobio.2016.06.010.
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Abstract
This study aimed to evaluate the performance of the Unyvero P50 pneumonia assay, the first ‘sample-in, answer-out’ system for rapid identification of pathogens and antibiotic resistance markers directly from clinical specimens. Overall, Unyvero P50 displayed very good sensitivity (>95%); however, specificity was low (33%) mainly because 40% of the specimens were reported as normal flora. Specifically, one or more pathogens were identified in 28 of them. From a detailed analysis of 42 specimens selected at random, 76% of the additionally reported pathogens were confirmed present in primary specimens. Detection of selected resistance markers was compared to routine phenotypic susceptibility testing, supplemented with Checkpoints microarray system, PCR and sequencing. Concordance was mixed, primarily due to issues with panel’s choice of markers and detection of some intrinsic beta-lactamases. Finally, we offer a critical analysis of the assay’s microbial panel and resistance markers and provide suggestions for improvement.
| Type: | Article |
|---|---|
| Title: | 'Sample-in, answer-out'? Evaluation and comprehensive analysis of the Unyvero P50 pneumonia assay |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.diagmicrobio.2016.06.010 |
| Publisher version: | http://dx.doi.org/10.1016/j.diagmicrobio.2016.06.0... |
| Language: | English |
| Additional information: | © 2016. This manuscript version is published under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International licence (CC BY-NC-ND 4.0). This licence allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licences are available at http://creativecommons.org/licenses/by/4.0. Access may be initially restricted by the publisher. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Infectious Diseases, Microbiology, Pneumonia, Rapid Diagnostics, Antimicrobial Resistance, Beta-Lactamase, Pcr, Hospital-Acquired Pneumonia, Antimicrobial Resistance, Multiplex Pcr, Management, Genes, Guidelines, Evolution, Bacteria, Outcomes, Europe |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery-pp.ucl.ac.uk/id/eprint/1504015 |
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